Western Blotting

      I've got a question for everyone out there familiar with some molecular biology techniques. Could you explain western blotting? I understand the application, and I understand the procedure up to the point where you blot your protein bands onto nitrocellulose or the like. After that, it starts to get a bit hazy for me.
      I understand the use of a probe to isolate your protein of interest, and that the probe has to be attached to some other protein which is itself fluorescent or is an enzyme which cleaves a substrate into something that fluoresces/dyes so identification can occur.
      However, I don't get how someone selects the specific antibody probe, or how you image the damn thing afterwards. Are there just a few, select antibody groups that bind to some tags (6xHIS, etc.), or what? And is there a way to isolate your protein of interest if you haven't tagged its sequence with an AA marker? Like, if you didn't modify the CDS of the gene that produced that protein's mRNA so it had a polyhistidine tag, is there an antibody you could still use based on the protein's understood properties? Any help/explanation would be appreciated.

>>1

Freak!

>>2

Dumbass.

She shrieked, and wriggled down on to the floor. I tried to mount her there. She kicked, fought, so that though once my prick touched her cunt-wig, I could not keep on the saddle. She forgot all propriety in her fuddled excitement, and whilst screaching from my tickling, repeated incoherently baudy words as I uttered them. "Let me fuck you." "You shan't fuck me." "Let's put it just to your cunt." "You shan't,—you're a blackguard,—oh! don't,—leave me alone,—wee I will feel it, if you'll let me get up,—oh!—he! hi! hi!—for God's sake don't tickle,—hi!—I shall go mad,—you shan't,—oh! don't,—oh I if you don't leave off." "I shall,—I must." "Oh! pray,—you shall if you leave off tickling then,—oh! don't pray,—oh! I shall piddle myself,—he! he!" She was rolling on the floor, her thighs exposed, sometimes backside, sometimes belly upwards with all its trimmings visible. "Oh! it's your fault," and as she spoke actually piddle began to issue. I had my hand on her thigh, and felt and saw it.

I suppose this is what happens when someone asks something legitimate of /sci/.

i love you

          ▄▄▄▄▄▄
         ▄▀█▀█▄██████████▄▄
        ▐██████████████████▌
        ███████████████████▌
       ▐███████████████████▌
       █████████████████████▄
    ▄█▐█▄█▀█████████████▀█▄█▐█▄
  ▄██▌██████▄█▄█▄█▄█▄█▄█████▌██▌
 ▐████▄▀▀▀▀████████████▀▀▀▀▄███
 ▐█████████▄▄▄▄▄▄▄▄▄▄▄▄██████▀
    ▀▀████████████████████▀
          ▀▀▀▀▀▀▀▀▀▀▀▀
  █▀▀ ▄ ▄  ▄  █    ▄  ▄ ▄  ▀   ▄▄
  █▀  █ █ █ █ █▀▄ █ █ █▀  ▀█  █ 
  ▀▀▀  ▀▀ █▀  ▀ ▀  ▀  ▀   ▀▀▀  ▀▀

>>5

"My fellow Americans, ask not what your country (or in this case the board) can do for you, ask what you can do for your country (or in this case the board)."

John F. Kennedy