PCR optimization

Anyone here do a lot of PCRs (Polymerase Chain Reactions)?  I was wondering what steps you take to optimize your PCR, what kind of gradients you do, and how many PCRs it generally takes you to get to your final optimized conditions.

If it matters, you're PCRing up a plasmid, and you're also PCRing an insert out of a bacterial genome.

>>2
Your methods ar fail.
First of all, If you keep your primer constant your BSA has no choice but to remain stagnant, which is not ideal.
Try using a fluctuating DMSO and a stable CoA.